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. 2021 Apr 27;186(4):2169–2189. doi: 10.1093/plphys/kiab190

Figure 1.

Figure 1

Phenotypes associated with CaNAC2c silencing in pepper plants and overexpression in N. benthamiana plants on thermotolerance and resistance to RSI (R. solanacearum infection). A, CaNAC2c-silenced pepper plants display reduced basal (BT) and acquired (AT) thermotolerance (pretreated with 37°C for 12 h and recovery for 12 h). B, Plant mortality (24 plants were calculated) exposed to HTS from 1 to 42 h post-treatment (hpt). C, Fv/Fm and △F/Fmʹ in leaves of CaNAC2c-silenced pepper plants challenged with HTS. D, Fv/Fm and △F/Fmʹ in leaves of CaNAC2c-silenced pepper plants challenged with HTS by dipping half of the leaf in water set to 42°C. E, Increased resistance of N. benthamiana plants overexpressing CaNAC2c to RSI relative to control plants. F and G, Simultaneous silencing of CaNAC2c and CaNAC2d produces an additive decrease in the resistance of pepper plants to RSI (24 plants were calculated for disease index). H, Height of CaNAC2c-silenced and control pepper plants. I, Root length of CaNAC2c-silenced and control pepper plants. J, Overall morphology of CaNAC2c-silenced and control pepper plants 35 d after sowing. In E, F, and J, the images were digitally extracted. In (C, D, G, H, and I), data presented are means ± standard error (SE) of four replicates, different capital letters indicate significant differences among means (P < 0.01), as calculated with Fisher’s protected LSD test.