Figure 2.

CaNAC2c Resistance to RSI is Repressed upon HTS. A and B, Activity of the CaNAC2cpro:GUS reporter to the application of SA (2 mM), ABA (30 μM), MeJA (10 μM), fluridon (10 μM) or HTS. Agrobacterium cells carrying the CaNAC2cpro:GUS reporter were infiltrated into pepper plants leaves, and GUS activity measured after HTS, fluridon, or phytohormones at 24 hpt (CK: pepper plants leaves infiltrated with GV3101 cells carrying pMDC-163). C, Effect of exogenous application of ABA on-resistance of CaNAC2c overexpressing N. benthamiana plants to Ralstonia solanacearum infection. D, Effect of the exogenously applied fluridon on acquired thermotolerance of CaNAC2c overexpressing N. benthamiana plants. E, Effect of exogenous application of ABA on thermotolerance of CaNAC2c-silenced pepper plants. F, HR-like cell death lesions at room temperature or under HTS triggered by transient overexpression of CaNAC2c or exogenous application of fluridon or ABA. G, H₂O₂ accumulation, displayed by DAB staining in CaNAC2c transiently overexpressing pepper leaves challenged with RSI, HTS, or with fluridon under HTS. H, H₂O₂ accumulation in CaNAC2c transiently overexpressing pepper leaves challenged with RSI, ABA, HTS, or with fluridon under HTS (F: fluridon). I, Transcript level of CaDEF1, CaCOI1, CaLOX14, CaLOX31, CaHIR1, CaHSP24, CaAPX, or CaABI5 in CaNAC2c transiently overexpressing pepper leaves challenged with RSI, ABA, or with ABA under RSI. J, Transcript level of CaDEF1, CaCOI1, CaLOX14, CaLOX31, CaHIR1, CaHSP24, CaAPX, or CaABI5 in CaNAC2c transiently overexpressing pepper leaves challenged with HTS or with fluridon under HTS (F: fluridon). In C–E, the images were digitally extracted. Data presented are means ± standard error (SE) of four replicates. In A, B, H, I, and J, different capital letters indicate significant differences among means (P < 0.01), as calculated with Fisher’s protected LSD test.