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. 2021 Apr 27;186(4):2169–2189. doi: 10.1093/plphys/kiab190

Figure 7.

Figure 7

Effect of CaHSP70 silencing on HR-like cell death and immunity and expression of thermotolerance-related genes induced by transient overexpression of CaNAC029. A, Stability of CaNAC2c-myc in pepper plants transiently overexpressing CaNAC2c-myc at room temperature or upon HTS. Time since 10 is shown in min. B, Effect of CaHSP70 silencing on the stability of CaNAC2c in pepper plants at room temperature or upon HTS. C, CaNAC2c was protected from degradation by CaHSP70 through in vitro assay using specific inhibitors (200 μM Apoptozole) of CaHSP70. D, Stability of CaNAC029-HA in pepper plants transiently overexpressing CaNAC029-HA at room temperature or upon HTS. Time since 10 is shown in min. E, Effect of CaHSP70 silencing on the stability of CaNAC29-HA in pepper plants transiently overexpressing CaNAC20-HA at room temperature or upon HTS. F, Immunoblot analysis of CaNAC29-HA in pepper plants transiently overexpressing CaNAC29-HA upon HTS with an anti-Ub antibody. G, Effect of CaHSP70 silencing on the HR-like cell death response triggered by the transient overexpression of CaNAC29 at room temperature and upon HTS.