Figure 2.

Double staining of peritubular macrophages with major histocompatibility complex class II (MHCII; green) and CD68 (red) are shown at all treatment and age endpoints; (A, B 48 h after mono-(2-ethylhexyl) phthalate (MEHP) exposure, (C, D) 1 week after MEHP exposure, and (E, F) 2 weeks after MEHP exposure. The Hoescht (blue) nuclei counter stain is used in all images and the scale bar shown in (A) is the same for all images. All MHCII⁺ macrophages are also CD68⁺ regardless of treatment, age, or time after exposure. All imaged were taken at 63×. The scale bar (50 μm) represented in (A) applies to all images in this figure.