Figure 3.

AtABCG14 disruption in the shoot reduces cytokinin signaling. A, GUS staining of flower inflorescence (top) and rosette leaves (bottom) of grafted plants WT/WT, atabcg14/atabcg14, WT/atabcg14, and atabcg14/WT at 35 DAG. Scale bar = 1 mm. B and C, Expression levels of type-A cytokinin response genes (ARR4, ARR5, ARR6, ARR7, ARR15, and ARR16) in the shoots (B) and roots (C) of the grafted plants at 25 DAG. The transcript level of the target gene was normalized to that of ACTIN2. Data are presented as mean ± sd (n = 4 biological replicates; representative data from two independent experiments). Statistical analyses were performed using the one-way analysis of variance (LSD test) with SPSS software (v. 13.0). Different letters above each column indicate significant differences (P < 0.05).