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. 2021 May 12;186(4):1770–1785. doi: 10.1093/plphys/kiab220

Figure 2.

Figure 2

Field-deployable CRISPR/Cas-based detection of plant viruses. Nucleic acid (DNA, or RNA) isolated from plants is subjected to isothermal amplification (loop-mediated isothermal amplification, LAMP or recombinase polymerase amplification, RPA for DNA viruses or reverse transcriptase coupled loop-mediated isothermal amplification, RT-LAMP, reverse transcriptase coupled recombinase polymerase amplification, RT-RPA for RNA viruses). Upon specific recognition and digestion of virus nucleic acid, Cas12a nonspecifically degrades an ssDNA reporter and releases the quenched fluorescence. End-point fluorescence (color change) is visualized with the naked eye using a P51 fluorescence lightbox.