Table 1.
Summary of CRISPR/Cas-mediated virus resistance
| CRISPR/Cas system used | Targeted plant virus | Plant used | Main observation of the study | References |
|---|---|---|---|---|
| Sp-CRISPR/Cas9 | TYLCV | Nicotiana benthamiana, Tomato (Lycopersicon esculentum) | CRISPR/Cas9 validation for plant virus resistance / Virus genome cutting | (Ali et al., 2015b; Tashkandi et al., 2018) |
| Sp-CRISPR/Cas9 | BSCTV | Nicotiana benthamiana | CRISPR/Cas9 validation for plant virus resistance / Virus genome cutting | (Ji et al., 2015) |
| Sp-CRISPR/Cas9 | BeYDV | Nicotiana benthamiana | CRISPR/Cas9 validation for plant virus resistance / Virus genome cutting | (Baltes et al., 2015) |
| Sp-CRISPR/Cas9 | MeMV | Nicotiana benthamiana | Multiple virus targeting with single sgRNA | (Ali et al., 2016) |
| Sp-CRISPR/Cas9 | BCTV. | Nicotiana benthamiana | Multiple virus targeting with single sgRNA | (Ali et al., 2016) |
| Sp-CRISPR/Cas9 | CLCuMV | Nicotiana benthamiana | Multiplex targeting and virus escape, Replication inhibition | (Ali et al., 2016; Khan et al., 2019; Yin et al., 2019) |
| Sp-CRISPR/dCas9 | ||||
| Sp-CRISPR/Cas9 | ChLCV | Nicotiana benthamiana | Multiplex targeting and virus escape | (Roy et al., 2019) |
| Sp-CRISPR/Cas9 | CaMV | Arabidopsis thaliana | Multiplex targeting and virus escape | (Liu et al., 2018) |
| Sp-CRISPR/Cas9 | endogenous eBSV | banana (Musa spp.) | Multiplex targeting and virus escape | (Tripathi et al., 2019) |
| Sp-CRISPR/Cas9 | WDV | barley (H. vulgare L. cv Golden promise) | Multiplex targeting of overlapping sequences of different genes in the virus genome | (Kis et al., 2019b) |
| Sp-CRISPR/Cas9 | ACMV | Nicotiana benthamiana | virus escape | (Mehta et al., 2019) |
| Fn-CRISPR/Cas9, Fn-CRISPR/dCas9 | TMV | Nicotiana benthamiana | Replication inhabitation of RNA virus | (Zhang et al., 2019) |
| Fn-CRISPR/Cas9, Fn-CRISPR/dCas9 | CMV | Nicotiana benthamiana | Replication inhabitation of RNA virus | (Zhang et al., 2019) |
| Lsh-CRISPR/Cas13a | TuMV | Nicotiana benthamiana, Arabidopsis thaliana | RNA virus genome targeting | (Aman et al., 2018a, 2018b, Zhan et al., 2019) |
| CRISPR/Cas13 | PVY | Potato (S. tuberosum) | RNA virus genome targeting, Multiple strains targeting, broad-spectrum resistance | (Zhan et al., 2019) |
| Lsh-CRISPR/Cas13a | Southern rice black-streaked draft virus (SRBsDV) | Rice (Oryza sativa) | RNA virus genome targeting | (Zhan et al., 2019) |
|
TuMV | Nicotiana benthamiana | Characterization for robust CRISPR/Cas system for RNA virus genome targeting | (Mahas et al., 2019) |
| Sp-CRISPR/Cas9 | Cucumber vein yellow virus | Cucumber (C. sativus L.) | Host genome (eIF4E) modification for broad-spectrum resistance to potyviruses | (Chandrasekaran et al., 2016) |
| Sp-CRISPR/Cas9 | CuVYV), Zucchini yellow mosaic virus (ZYMV) | Cucumber (C. sativus L.) | Host genome (eIF4E) modification for broad-spectrum resistance to potyviruses | (Chandrasekaran et al., 2016) |
| Sp-CRISPR/Cas9 | Papaya ring spot virus-W (PRSV-W) | Cucumber (C. sativus L.) | Host genome (eIF4E) modification for broad-spectrum resistance to potyviruses | (Chandrasekaran et al., 2016) |
| Sp-CRISPR/Cas9 | TuMV | Arabidopsis thaliana | Host genome (eIF4E) modification for resistance to RNA viruses | (Pyott et al., 2016) |
| Sp-CRISPR/Cas9 | Rice tungro spherical virus (RTSV) | Rice (O. sativa) | Host genome (eIFiso4E) modification for broad-spectrum resistance to RNA viruses | (Macovei et al., 2018) |
| Sp-CRISPR/nCas9-base editor | Clover yellow vein virus (ClYVV) | Arabidopsis thaliana | Mimicking natural resistance alleles elF4E (N176K; amino acid substitutions) | (Bastet et al., 2019) |
| Sp-CRISPR/Cas9 | Cassava brown streak virus (CBSV) | Cassava plant (Manihot esculenta Crantz) | Host genome (eIF4E isoforms nCBP‐1 and nCBP‐2) modification for broad-spectrum resistance to RNA viruses | (Gomez et al., 2019) |
| Sp-CRISPR/Cas9 | PVY | Potato (S. tuberosum) | Host genome (Coilin) modification for broad-spectrum resistance to RNA viruses | (Makhotenko et al., 2019) |
| Sp-CRISPR/Cas9 | Soya bean mosaic virus (SMV) | soya bean [Glycine max (L.) Merr.] | Host genome (GmF3H1, GmF3H2, and GmFNSII-1, members of the metabolites Isoflavonoids pathway) modification for broad-spectrum resistance to RNA viruses | (Zhang et al., 2020) |