Table 2.
Summary of virus vectors for CRISPR/Cas machinery delivery into plant
| Plant virus vector | Genome | Target plant | Main goal of virus-based delivery | Reference/s |
|---|---|---|---|---|
| BeYDV replicons | DNA |
|
Virus replicons development for CRISRP/Cas system delivery, for host genome editing and HDR-based repair, gene targeting | (Baltes et al., 2014; Butler et al., 2016; Čermák et al., 2017; Dahan-Meir et al., 2018) |
| Cabbage leaf curl virus (CaLCuv) | DNA | Nicotiana benthamiana | VIGE (Virus induce genome editing), systemic delivery of sgRNA to increase the recovery of edited regenerated plants | (Yin et al., 2015) |
| WDV | DNA |
|
Virus replicons development for CRISRP/Cas system delivery and HDR based repair | (Čermák et al., 2017; Wang et al., 2017) |
| Cotton leaf crumple virus (CLCrV) | DNA | Arabidopsis thaliana | sgRNAs delivery stem apical meristem and germline cells | (Lei et al., 2021) |
| TRV | RNA | Nicotiana benthamiana | Systemic delivery of sgRNA, sgRNA-FT, sgRNA-tRNA to invade germline cells to increase the recovery of edited plants, to obviate tissue culture | (Ali et al., 2015a, 2015b, 2016; Aman et al., 2018a, 2018b; Mahas et al., 2019; Ellison et al., 2020) |
| PEBV | RNA | Nicotiana benthamiana, Arabidopsis thaliana | Systemic delivery of sgRNA to increase the recovery of edited plants | (ALi et al., 2018) |
| TMV | RNA | Nicotiana benthamiana | To enhance systemic delivery of sgRNA to increase the recovery of edited plants, sgRNAs processing | (Cody and Scholthof, 2020) |
| Beet necrotic yellow vein virus (BNYVV) | RNA | Nicotiana benthamiana | Multiple sgRNAs delivery | (Jiang et al., 2019) |
| Foxtail mosaic virus | RNA | Foxtail (Setaria viridis), Maize (Z. mays) | sgRNAs delivery to monocots | (Mei et al., 2019) |
| Barley yellow striate mosaic virus (BYSMV) | RNA | Nicotiana benthamiana | Delivery of the whole CRISPR/Cas system to plant tissue to increase the recovery of foreign DNA free genome-edited plants | (Ma et al., 2020) |
| Sonchus yellow net rhabdovirus (SYNV) | RNA | Nicotiana benthamiana | Delivery of the whole CRISPR/Cas system to plant tissue to increase the recovery of foreign DNA free genome-edited plants | (Ma et al., 2020) |
| PVX | RNA | Nicotiana benthamiana | Delivery of tandemly arranged sgRNAs for simultaneous multisite genome editing and the whole CRISPR/Cas system to plant tissue to increase the recovery of foreign DNA free genome-edited plants | (Ariga et al., 2020; Uranga et al., 2021) |