Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK 293 | ATCC | CRL-1573 | Embryonic kidney |
| Cell line (Mus musculus) | 3T3-L1 | ATCC | CL-173 | The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent state |
| Primary fibroblasts | T1 | Pr. Fève lab at CRSA, Paris | N/A | Non-obese and non-diabetic female, skin biopsy |
| Primary fibroblasts | T2 | Pr. Fève lab at CRSA, Paris | N/A | Non-obese and non-diabetic female, skin biopsy |
| Primary fibroblasts | Patient 1 | Pr. Fève lab at CRSA, Paris | N/A | Patient 1, female, skin biopsy |
| Adipose stem cells | ASCs | Pr. Fève lab at CRSA, Paris | N/A | Female, from subcutaneous abdominal adipose tissue |
| Antibody | Anti-adiponectin | Thermo Fisher Scientific | Cat# MA1-054 | WB (1:1000) |
| Antibody | Anti-AKT | Santa Cruz Biotechnology | Cat# sc-8312 | WB (1:1000) |
| Antibody | Anti β-actin | Sigma Aldrich | Cat# A2228 | WB (1:10,000) |
| Antibody | Anti-Calnexin | ENZO Life Science | Cat# ADO-SPA-860 | IF (1:200) |
| Antibody | Anti-C/EPBα | Protein Tech | Cat# 18311-1-1P | WB (1:1000) |
| Antibody | Anti-EPHX1 | Novus | Cat# NBP1-3301 | WB (1:1000) - IF (1:1000) |
| Antibody | Anti-ERK | Cell Signaling Technology | Cat# 9102 | WB (1:1000) |
| Antibody | Anti-FAS | Cell Signaling Technology | Cat# 3180 | WB (1:1000) |
| Antibody | Anti-Flag | Origene | Cat# TA50011-100 | WB (1:1000) - IF (1:1000) - IP (1:200) |
| Antibody | Anti-IRΒ | Cell Signaling Technology | Cat# 3025 | WB (1:1000) |
| Antibody | Anti-IRS1 | Protein Tech | Cat# 17509–1-AP | WB (1:1000) |
| Antibody | Anti-P16 | Protein Tech | Cat# 10883–1-AP | WB (1:1000) |
| Antibody | Anti-P21 | Protein Tech | Cat# 10355–1-AP | WB (1:1000) |
| Antibody | Anti-P53 | Abcam | Cat# ab1101 | WB (1:1000) |
| Antibody | Anti-P-AKT | Santa Cruz Biotechnology | Cat# sc-7985-R | WB (1:1000) |
| Antibody | Anti-P-ERK | Cell Signaling Technology | Cat# 9101 | WB (1:1000) |
| Antibody | Anti-P-P53 | Abcam | Cat# ab38497 | WB (1:1000) |
| Antibody | Anti-PPARg | Protein Tech | Cat# 16643–1-AP | WB (1:1000) |
| Antibody | Anti-SREBP-1 | Santa Cruz Biotechnology | Cat# sc-366 | WB (1:1000) |
| Antibody | Anti-Tubulin | Protein Tech | Cat# 66031–1-lg | WB (1:10,000) |
| Antibody | Anti-P-Tyr | Santa Cruz Biotechnology | Cat# sc-7020 | WB (1:1000) |
| Antibody | Anti-rabbit-HRP | GE Healthcare | Cat# NA934V | WB (1:2000) |
| Antibody | Anti-mouse-HRP | GE Healthcare | Cat# NA931V | WB (1:2000) |
| Recombinant DNA reagent (plasmid) | pCMV-entry-Flag | Origene | Cat # PS100001 | |
| Recombinant DNA reagent (plasmid) | pCMV-EPHX1 WT-Flag or without Flag | This paper | N/A | Described in Materials and methods Publicly available (Addgene) |
| Recombinant DNA reagent (plasmid) | pCMV-EPHX1 c.337T>C -Flag or without Flag | This paper | N/A | Described in Materials and methods Publicly available (Addgene) |
| Recombinant DNA reagent (plasmid) | pCMV-EPHX1 c.416A>G -Flag or without Flag | This paper | N/A | Described in Materials and methods Publicly available (Addgene) |
| Recombinant DNA reagent (plasmid) | pCMV-EPHX1 c.997A>C -Flag or without Flag | This paper | N/A | Described in Materials and methods Publicly available (Addgene) |
| Recombinant DNA reagent (plasmid) | pCMV-EPHX1 c.1212G>C -Flag or without Flag | This paper | N/A | Described in Materials and methods Publicly available (Addgene) |
| Recombinant DNA reagent (plasmid) | pCMV-EPHX1 c.1288G>C -Flag or without Flag | This paper | N/A | Described in Materials and methods Publicly available (Addgene) |
| Recombinant DNA reagent (plasmid) | pSpCas9(BB)−2A-GFP (PX458) | Addgene | Cat# 48138 | A gift from Zhang lab |
| Recombinant DNA reagent (plasmid) | pLentiCRISPR v2 | Addgene | Cat# 52961 | A gift from Zhang lab |
| Software algorithm | FIJI software | NIH | N/A | |
| Software algorithm | GraphPad | Graphpad Software | N/A | |
| Software algorithm | Prism | Graphpad Software | N/A |