Figure 3.

miR-140-3p inhibited cell proliferation, migration, invasion, and EMT via targeting ANXA8

Mimics NC, miR-140-3p mimics, miR-140-3p mimics+pcDNA3.1, or miR-140-3p mimics+pcDNA3.1-ANXA8 were transfected into T24 and UMUC-3 cells. (A) The mRNA level of ANXA8 was determined by qRT-PCR. (B and C) The ANXA8 expression was assessed by western blotting with quantitative analysis. (D and E) Clonogenic ability was assessed by colony formation assay with quantitative analysis. (F and G) Cell apoptotic rate was assessed by flow cytometry with quantitative analysis. (H–K) The migratory (H and I) and invasive (J and K) capacities were examined by transwell migration and invasion assays with quantitative analysis. Scale bar, 100 μm. (L and M) The expressions of EMT markers were assessed by western blotting with quantitative analysis. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.