Figure 5.

Silencing of miR-140-3p or ANXA8 overexpression reversed the effects of TUG1 knockdown on bladder cell proliferation and apoptosis

T24 and UMUC-3 cells were transfected with shNC, shTUG1, shTUG1+inhibitor NC, shTUG1+miR-140-3p inhibitor, shTUG1+pcDNA3.1, or shTUG1+pcDNA3.1-ANXA8. (A and B) Colony-forming ability was determined by colony formation assay with quantitative analysis. (C and D) Cell apoptotic rate was assessed by flow cytometry with quantitative analysis. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.