Figure 6.

Silencing of miR-140-3p or ANXA8 overexpression reversed the effects of TUG1 knockdown on bladder cell migration, invasion, and the EMT process

T24 and UMUC-3 cells were transfected with shNC, shTUG1, shTUG1+inhibitor NC, shTUG1+miR-140-3p inhibitor, shTUG1+pcDNA3.1, or shTUG1+pcDNA3.1-ANXA8. (A–D) The migratory (A and B) and invasive (C and D) capacities were examined by transwell migration and invasion assays with quantitative analysis. Scale bar, 100 μm. (E and F) The EMT marker level was assessed by western blotting with quantitative analysis. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.