The expression of stemness markers analyzed by RT-qPCR. Expression of stemness markers NANOG, KLF4, SOX2 and OCT3/4 in miPSCs, miPS-T47Dcm cells, tumors Tm1, Tdm, Ts.c., Tds.c. of miPS-T47Dcm cell transplants, miPS-BT549cm cells, tumors Bm1, Bdm, Bs.c., Bds.c. of miPS-BT549cm cell transplants (from left to right). Each bar in the graphs represents the average of three reactions (mean ± SD). The significance was compared with the expression in miPSCs. *P<0.05, **P<0.01, ***P<0.001.