REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
BV421 anti-human CD196 (CCR6, clone 11A9)	BD Horizon	562515
efluor450 anti-human IL17A (clone eBio64DEC17)	Invitrogen	48-7179-42
BV480 anti-human CD127 (clone HIL-7R-M2)	BD Horizon	566101
efluor506 anti-human CD14 (clone 61D3)	Invitrogen	69-0149-42
BV570 anti-human CD45RA (clone HI100)	BioLegend	304132
BV605 anti-human CD16 (clone 3G8)	BioLegend	302040
BV650 anti-human TNF-alpha (clone Mab11)	BioLegend	502938
Qdot655 anti-human CD27 (clone CLB-27/1)	Invitrogen	Q10066
BV711 anti-human OX40 (clone ACT35)	BioLegend	350030
BV750 anti-human CD3 (clone SK7)	BioLegend	344846
BV785 anti-human CCR7 (clone G043H7)	BioLegend	353230
FITC anti-human CD154 (clone 24-31)	Invitrogen	11-1548-42
AF532 anti-human CD4 (clone RPA-T4)	Invitrogen	58-0049-42
PercP anti-human HLA-DR (clone L243)	BioLegend	307628
PercP-Cy5.5 anti-human CCR4 (clone 1G1)	BD PharMingen	560726
PercP-efluor710 anti-human CD8 (clone SK1)	Invitrogen	46-0087-42
PE anti-human IL2 (clone MQ1-17H12)	Invitrogen	12-7029-42
PE-efluor610 anti-human CXCR3 (clone CEW33D)	Invitrogen	61-1839-42
PE-Cy5 anti-human CD137 (clone 4B4-1)	BioLegend	309808
PE-Cy7 anti-human CD69 (clone FN50)	BioLegend	310912
APC anti-human IL4 (clone MP4-25D2)	BioLegend	500812
AF647 anti-human CXCR5 (clone RF8B2)	BD PharMingen	558113
AF700 anti-human IFN-gamma (clone B27)	BD PharMingen	557995
APC-efluor780 anti-human CD25 (clone BC96)	Invitrogen	47-0259-42
APC-Fire810 anti-human CD19 (clone HIB19)	BioLegend	302272
Biological samples
Peripheral blood mononuclear cells (PBMCs)	Patients and healthy donors	N/A
Specific antibodies measured in serum samples	Patients and healthy donors	N/A
IFN-gamma measured in whole blood samples	Patients and healthy donors	N/A
Chemicals, peptides, and recombinant proteins
Zombie NIR Fixable Viability Kit	BioLegend	423106
Ficoll-Paque Plus	GE Healthcare	GE17-1440-02
AIM-V medium	GIBCO	12055091
Human AB serum	Gemini Bio-Products, Inc.	100-512
GolgiPlug (Protein Transport Inhibitor)	BD Biosciences	555029
SARS-CoV-2 peptide pools of 15-mers (55 peptides)	Le Bert et al., 2021	N/A
PEPTIVATOR SARS-COV-2 PROT_S+, RG 60NMOL	MILTENY	130-127-312
PEPTIVATOR SARS-COV-2 PROT_S, RG 60 NMOL	MILTENY	130-126-701
PEPIVATOR SARS-COV-2 PROT_S1,RG 60 NMOL	MILTENY	130-127-048
Critical commercial assays
Access SARS-CoV-2 IgG Antibody Test	Beckman Coulter Inc	C58961
Simple Plex Human IL-2 Cartridge	ProteinSimple	SPCKB-PS-000295
Simple Plex Human IFN-gamma (3rd Gen) Cartridge	ProteinSimple	SPCKB-PS-002574
Deposited data
Raw and analyzed data	This paper	N/A
Software and algorithms
Flow data were collected with SpectroFlo® Software	Cytek Biosciences	N/A
Flow cytometry data were analyzed on Cytobank	https://cytobank.org/	N/A
Statistical analyses using Graphpad Prism (v9)	Graphpad Holdings, LLC	N/A