. 2021 Jul 13;10(7):401–410. doi: 10.1302/2046-3758.107.BJR-2020-0200.R2

Table I.

Oligonucleotide primer sequences for real-time quantitative reverse transcription polymerase chain reaction.

Gene	Primer direction	Sequence (5′--3′)
PARP-1	Forward	TCCCAGAACAAGGACGAAGC
PARP-1	Reverse	CCTCACACACGACTCGAACA
Aggrecan	Forward	ATGATGGCGCTGTTCTGAAGG
Aggrecan	Reverse	GAAGTGATGCATGGCATTGAGG
Col2A1	Forward	GGCAATAGCAGGTTCACGTACA
Col2A1	Reverse	GATAACAGTCTTGCCCCACTTACC
MMP-1	Forward	ACAGTTTCCCCGTGTTTCAG
MMP-1	Reverse	CCCACACCTAGGTTTCCTCA
MMP-3	Forward	TCTTTCACTCAGCCAATGCT
MMP-3	Reverse	GGGAGGTCCATAGAGGGATT
IL-6	Forward	GGATACCACCCACAACAGAC
IL-6	Reverse	TTGCCGAGTAGACCTCATAG
IL-1β	Forward	TCATTGTGGCTGTGGAGAAG
IL-1β	Reverse	CTATGTCCCGACCATTGCTG
TNF-α	Forward	CCCCTTTATCGTCTACTCCTC
TNF-α	Reverse	GCTGGTAGTTTAGCTCCGTTT
iNOS	Forward	GCATCCCAAGTACGAGTGGT
iNOS	Reverse	GAAGGCGTAGCTGAACAAGG
COX-2	Forward	GTGGGATGACGAGCGACTGT
COX-2	Reverse	TTTCAGGGAGAAGCGTTTGC
GAPDH	Forward	GAAGGTGAAGGTCGGAGTC
GAPDH	Reverse	GAAGATGGTGATGGGATTTC

Col2A1, collagen type II alpha 1 chain; COX-2, cyclooxygenase-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ICAM-1, intercellular cell adhesion molecule-1; IL, interleukin; iNOS, inducible nitric oxide synthase; MMP, matrix metalloproteinase; PARP-1, poly(ADP-ribose) polymerase-1; TNF-α, tumour necrosis factor alpha.