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. 2021 Aug 3;11:15691. doi: 10.1038/s41598-021-94401-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Diffusion coefficient (D) distributions of bulk water in the fibrinogen control and the purified fibrinogen solutions oxidized by increasing HOCl concentrations. (b) T₂ distributions of bulk water in the fibrinogen control and the purified fibrinogen solutions oxidized by increasing HOCl concentrations. Diffusion/T₂ correlations of water protons in the purified fibrinogen solutions oxidized by increasing HOCl concentrations (25, 50, 75, 100, 150 µM). The D/T₂ correlations were simultaneously measured by PFG for diffusion coefficient (D) and conventional CPMG for T₂ relaxation time. Fibrinogen solution without oxidation (0 µM HOCl) was used as a fibrinogen control. (c) T₁ distributions of bulk water in the plasma fibrinogen solution and plasma fibrinogen solution added with an increasing percentage of HOCl-oxidized fibrinogen (10, 20, 30, 40, 50%) (pre-oxidation of purified fibrinogen by 150 µM HOCl). (d) T₂ distributions of bulk water in the plasma fibrinogen solution and plasma fibrinogen solution added with an increasing percentage of HOCl-oxidized fibrinogen. Plasma without HOCl-oxidized fibrinogen (0%) was used as control plasma. Plasma fibrinogen solution was indirectly oxidized by adding HOCl-oxidized fibrinogen (10, 20, 30, 40, 50%). The T₁/T₂ correlations were simultaneously measured by saturation inversion recovery for T₁ time and conventional CPMG for T₂ relaxation time. (e) The correlation of bulk water T₂ relaxation time and the level of indirect oxidation of plasma fibrinogen solution added with an increasing percentage of HOCl-oxidized fibrinogen as well as the correlation of bulk water T₂ relaxation time and the level of indirect oxidation of plasma fibrin gels formed by increasing percentage of HOCl-oxidized fibrinogen. The correlations of bulk water T₂ relaxation times and the level of indirect oxidation in plasma fibrinogen solutions or plasma fibrin gels were analyzed by Pearson correlations. P < 0.05 is considered as a significant correlation. The 2D-NMR diffusion/T₂ correlations and T₁/T₂ were measured using 600 MHz NMR spectrometry. The multiple D/T₂ or T₁/T₂ water signals were analyzed by 2D inverse Laplace transform algorithm (2DILT) and Iterative Thresholding Algorithm for Multiexponential Decay (ITAMeD).