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. 2021 Jul 7;53(7):1134–1147. doi: 10.1038/s12276-021-00645-4

Fig. 5. Calbindin regulates the cell-surface localization of Kv4.1 via CaMKII-mediated phosphorylation.

Fig. 5

a, b Cell-surface biotinylation assay of control and shRNA calbindin (a) or calbindin (b)-transfected HT22 cells followed by immunoblot analysis with the indicated antibodies. c Analysis of serine phosphorylation of Kv4.1 in 293T cells expressing control or Kv4.1 and calbindin treated with 20 µM KN-92 or KN-93 for 24 h. d Quantification of the relative phosphorylation levels of Kv4.1 at serine residues shown in (c). The intensities were normalized to the level of β-tubulin. Values are means of triplicate determinants (ANOVA Tukey). Letters indicate statistically distinct groups (P < 0.05). e Surface biotinylation assay of control and calbindin-depleted HT22 cells treated with vehicle or 10 µM FK506 for 1 h followed by immunoblot analysis with the indicated antibodies. f Quantification of the relative cell-surface protein levels of Kv4.1 shown in (e). The intensities were normalized to the level of β-tubulin. *P < 0.05, **P < 0.01 versus the control group; #P < 0.05 versus the shCB group.