Table 1.

Effect of TCDD on acyl-CoA levels.

Compound ID	Description	Cn	Score	Fold-Change (TCDD vs. Veh.)
				0.3 µg/kg	1 µg/kg	3 µg/kg	10 µg/kg	30 µg/kg
HMDB01070	Octanoyl-CoA	8	36.8	1.77 ± 0.63	0.04 ± 0.01	0.28 ± 0.04	0.06 ± 0.03	0.01 ± 0.01
HMDB02845	Hexanoyl-CoA	6	37.2	1.43 ± 0.47	0.08 ± 0.02*	0.36 ± 0.05	0.08 ± 0.02	0.03 ± 0.02*
HMDB01088	Butyryl-CoA	4	44.3	1.24 ± 0.33	0.17 ± 0.05*	0.57 ± 0.10	0.09 ± 0.01*	0.08 ± 0.04*
HMDB01206	Acetyl-CoA	2	52.1	0.95 ± 0.12	0.30 ± 0.05*	0.53 ± 0.07*	0.04 ± 0.01*	0.16 ± 0.06*
HMDB03949	Octenoyl-CoA	8	34	1.66 ± 0.19	1.14 ± 0.23	26.16 ± 25.31	114.31 ± 4.89*	139.82 ± 6.88*

Acyl-CoA levels were assessed using untargeted liquid chromatography tandem mass spectrometry. Mice (n = 4–5) were orally gavaged every 4 days for 28 days with sesame oil vehicle or TCDD. Fold-changes were calculated for each treatment group relative to the vehicle control group. Bold font and asterisks (*) denote statistical significance (p ≤ 0.05) determined using a one-way ANOVA with a Dunnett’s post-hoc analysis. Scores were determined by Progenesis with 60 being the maximum value and 0 being the minimum value. Scores ranging from 30 to 40 are based on mass error and isotope distribution similarity, while score > 40 are based on mass error, isotope distribution and fragmentation score. All annotated compounds have a score distribution averaging ~ 35.