Assessment of plasma EVs isolated from subjects with diabetes and atherosclerosis
(A) Details of clinical data of the cohort used in this study.
(B) Analyses of 9 individual fractions of plasma EVs separated by C-DGUC method. An equal volume (37.5 μL) of fraction samples was loaded for Western blot analysis.
(C–E) (C) Particle diameter distribution profiles for fraction 8 estimated by NTA. Particle concentrations (D) and size (E) were estimated by NTA using NanoSight.
(F) Protein concentration was measured by qubit assay. Statistical analysis was performed using one-way ANOVA and Sidak's multiple comparisons post-test. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, ∗∗∗∗p< 0.0001. Data are represented as mean ± SEM.