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. 2021 Jul 27;36(4):109449. doi: 10.1016/j.celrep.2021.109449

Figure 1.

Figure 1

Pcl and Bamy mutually exclude each other in King’s B medium

(A) Pairwise interaction time lapses between Bamy (left) and Pcl (right) on LB (top) and King’s B (bottom) media at 24, 48, 72, and 96 h. Scale bars, 5 mm.

(B) Time-lapse microscopy of the pairwise interaction between Pcl and Bamy during 126 h. Scale bars, 2 mm.

(C) Expansion rates of the Bamy and Pcl leading edges and distance between both strains during the interaction. The orange line represents the Pcl leading edge, and the green and black lines represent Bamy leading edges 1 and 2, respectively. Error bars represents SD. n = 3. The blue area represents the distance between the two populations during the entire interaction.

(D) Confocal laser scanning microscopy (CLSM) time-course experiment of the interaction area using propidium iodide (PI) to identify cell death. Bamy is fluorescently labeled with yellow fluorescent protein (YFP). Scale bars, 40 μm.