Skip to main content
. 2021 Jul 27;36(4):109449. doi: 10.1016/j.celrep.2021.109449

Figure 3.

Figure 3

Bacillaene is a bacteriostatic compound produced by Bamy that arrests Pcl growth

(A) Time-course pairwise interactions between Pcl and Bamy mutants in secondary metabolites bacillaene (Δbae), fengycin (ΔfenA), and difficidin (Δdfn) on King’s B medium at 24, 48, and 72 h. Scale bars, 5 mm.

(B) Measurement, in percentage, of the inhibition area compared with the WT interaction between Pcl and Bamy mutants at 72 h. Mean values of three biological replicates are shown, with error bars representing SEM. The same letters indicate differences that were not significant (a = 0.05), using a one-way ANOVA followed by a Dunnett’s test, n = 3.

(C) Time-lapse microscopy of the pairwise interaction between Pcl and Δbae during 48 h. Scale bars, 2 mm.

(D) Expansion rates of the Pcl leading edges in the interaction with WT Bamy (red line) and Δbae (green line) from 20 to 50 h. Error bars represents SD. n = 3.