Figure 4.

Mutations in Pcl FusA confer bacillaene resistance

(A) Pairwise interaction between Pcl and Bamy after interaction for 144 h. Magnification of the selected area permits visualization of Bamy microcolonies (yellow triangles) and Pcl subzones (green triangles) growing in the inhibition area. Scale bars, 5 mm.

(B) Time-lapse microscopy of the pairwise interaction between FusA^T680D and Bamy after 74 h. Scale bars, 2 mm.

(C) Measurement of the Pcl colony movement within 24 h of their arrival at the initial position of a Bamy colony. Mean values of three biological replicates are shown, with error bars representing SEM. The same letters indicate differences that were not significant (a = 0.05), using a one-way ANOVA followed by a Dunnett’s test. n = 3.

(D) Expansion rates of the WT Pcl leading edges during interaction with WT Bamy (blue line), Δbae (orange line), and the FusA^T680D leading edge expansion rate during interaction with Bamy from 12 to 43 h. Error bars represents SD. n = 3.

(E) Molecular docking between bacillaene and FusA reveals a putative binding site formed by Arg29, Arg89, and Asn272 (highlighted in red).

(F) Molecular docking between bacillaene and FusA^T680D reveals changes in the amino acids involved in the binding site (Asn81, Arg83, and Asn272). Residues highlighted in pink are shared with the WT model while residues in red are the changing residues.