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. 2021 Aug 3;12:4674. doi: 10.1038/s41467-021-24827-w

Fig. 2. Comparison between experiments and coarsening model.

Fig. 2

a, b Model schematic: HEI10 molecules (with 1D concentration c) (red) diffuse along SC (green), absorb into foci (yellow, with HE10 amounts Cn at rate α, escape foci onto SC at rate β (Eqs. (1–3)). Boundary, initial conditions (Eqs. (4–5)). Initial HEI10 amount at RI focus drawn from truncated normal distribution NT (mean C0, std dev σ, values restricted within ±3σ). The graph shows function f modulating initial HEI10 RI loading near bivalent ends. Table: model parameter values. c–h Comparison of late-stage bivalent cytology data (left) with simulations (right) (experimental data from 395 bivalents from 95 individual cells from 11 wild-type plants). Crossovers (COs) in the experimental data are defined as the positions of late-HEI10 foci. c Number of COs per bivalent. d–g CO positions along bivalents. (813 COs). d All COs. e Bivalents with single CO. f Stacked histograms for double COs, showing positions of 1st/2nd CO. g Triple COs, showing positions of 1st/2nd/3rd CO. h Distribution of spacing between adjacent COs. i Effect of varying initial RI density on CO number per bivalent relative to wild-type (data18: red crosses; model prediction: blue line). j Example simulation kymographs of HEI10 focus intensities for triple (top left), double (top right), single (bottom left) COs. Shading: loge focus intensity. Traces of foci intensity against time (bottom right), showing triple CO simulation (top left). k Analysis of double CO HEI10 focus intensities; experiments (left), simulations (right): On arbitrarily aligned SC, intensity of left CO, relative to sum of both intensities, against relative position (measured from left end) of the midpoint between the two COs (CO ‘catchment area’). Blue lines: linear-regression best-fits. l Histograms of intensities of CO HEI10 foci, relative to sum of all foci within a cell, colour-grouped according to CO number per bivalent (inset); experiments (left), simulations (right). Source data are provided as a source data file.