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. 2021 Aug 3;11:15701. doi: 10.1038/s41598-021-95209-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Correlations between pulmonary function and cfDNA in plasma. (A) The lowest p/f ratio measured during stay on the ICU and the cfDNA at ICU admission correlated significantly (r = -0.236; p = 0.021). Correlations were calculated with the Spearman’s rank-order correlation test. Correlations were considered significant if P < 0.05. (B) Change in level of cfDNA in COVID-19 patients on ICU and ventilator free days (VFD). The change in the level of cfDNA in the plasma of COVID-19 patients was calculated by subtracting the average cfDNA level during late (> day 6) from the average level during early days (day 1–5). The patients were divided by VFD = 0 or > 0. P-values were calculated between the VFD groups divided by survival with the Mann–Whitney U test. P-value were considered significant if p < 0.05;*.