Table 2.
Overview of MPP concentrations used for proliferation tests, flow cytometry, and ROS assays. For all particle sizes, a low and high concentration, depending on the particle size, was applied. Low concentration = < 1% coverage of the plate; medium concentration = 1–10% coverage of the plate; high concentration = 20–30% coverage of the plate.
| Particle size | Low concentration (particles/cell) | High concentration (particles/cell) |
|---|---|---|
| 0.2 µm | 10 | 100,000 |
| 0.5 µm | 10 | 10,000 |
| 1 µm | 10 | 1000 |
| 2 µm | 10 | 400 |
| 3 µm | 10 | 100 |
| 6 µm | 2 | 25 |