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. 2021 Jul 20;53(7):1170–1179. doi: 10.1038/s12276-021-00652-5

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© The Author(s) 2021, corrected publication 2022

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Fig. 3 — a Effects of LPS on the induction of S100A9 in PBNs. b ROS formation in PBNs under LPS/S100A9 stimulation. c The percentage of migrating PBNs under LPS/S100A9 stimulation. d The levels of IL-8 produced by PBNs under various conditions. e The formation of NETs as observed by using confocal microscopy. Scale bar, 25 μm. f The time-dependent phosphorylation of ERK and p38 in PBNs treated with S100A9. g NET levels were evaluated by extracellular DNA concentrations were measured using the PicoGreen assay and h using confocal microscopy. The data are presented as the means ± SD. *P < 0.050, **P < 0.010, ***P < 0.001 were obtained by one‐way ANOVA with Bonferroni’s post hoc test. DAPI 4′,6-diamidino-2-phenylindole, ERK extracellular signal-regulated kinase, HC healthy control, IL interleukin, LPS lipopolysaccharide, MPO myeloperoxidase, NE neutrophil elastase, NET neutrophil extracellular trap, NSA non-severe asthma, PMA phorbol 12-myristate 13-acetate, S100A9 S100 calcium-binding protein A9, SA severe asthma.