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. 2021 Aug 3;11:15688. doi: 10.1038/s41598-021-95342-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis shows changes in TNFα and IL10 protein expression relative to β-actin protein (control). (A) TNFα and IL10 protein bands. (B and C) Band quantification of TNFα and IL10 protein (ImageJ, https://imagej.nih.gov/ij/index.html). Data presented as fold change (mean) ± SEM (n = 7/group). Columns carrying different letters [a (the highest value) – d (the lowest value)] are significantly different at p < 0.05. All groups were compared to each other.