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. 2021 Aug 3;12(8):764. doi: 10.1038/s41419-021-04063-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A, B The mRNA levels of ANLN-201 and ANLN-210 in collected HNSCC tumor tissues and paired non-tumor tissues (n = 13) were analyzed by qRT-PCR. C, D ANLN-201 mRNA and ANLN-210 mRNA were detected in HNSCC tissues and several human cancer cell lines (BEAS-2B, human bronchial epithelial cells; FaDu, the hypopharyngeal carcinoma cell line; SCC-9 and SCC-15, the tongue squamous cell lines; SNU899, the laryngeal carcinoma cell line; HepG2, the hepatocellular carcinoma cell line; HCT-116, the colon cancer cell line; MCF-7, the breast cancer cell line) using Northern blot probes against ANLN-201 and ANLN-210. T: tumor tissues, NT: non-tumor tissues. E, F Kaplan–Meier plots of OS associated with ANLN-201 and ANLN-210 risk stratification in clinical trial cohort. G Kaplan–Meier plots of OS associated with ANLN risk stratification in external database.