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. 2021 Aug 3;12(8):764. doi: 10.1038/s41419-021-04063-2

Fig. 8. Alternatively spliced ANLN isoforms synergistically promote HNSCC tumor growth in vivo.

Fig. 8

A Representative images of HNSCC tumors from mice co-injected SCC-9 cells with THP-1 cells (induced by PMA) treated with exosomes (4:1) via the tail veins (n = 3). The groups were as follows. ① ANLN-sgRNA SCC-9 cells and THP-1 (induced by PMA) treated with PBS, ② ANLN-sgRNA SCC-9 cells and THP-1 (induced by PMA) treated with exosomes secreted from ANLN-sgRNA SCC-9 cells, ③ ANLN-sgRNA SCC-9 cells and THP-1 (induced by PMA) treated with ANLN-210 O.E. exosomes ④ ANLN-201-overexpressing SCC-9 cells and THP-1 (induced by PMA) cells treated with exosomes secreted from ANLN-sgRNA SCC-9 cells, ⑤ ANLN-201-overexpressing SCC-9 cells and THP-1 (induced by PMA) cells treated with ANLN-210 O.E exosomes, ⑥ ANLN-210-overexpressing SCC-9 cells and THP-1 (induced by PMA) cells treated with exosomes secreted from ANLN-sgRNA SCC-9 cells, and ⑦ ANLN-210-overexpressing SCC-9 cells and THP-1 cells (induced by PMA) treated with ANLN-210 O.E exosomes. B Representative immunohistochemistry analysis of the expression of CD163 and Ki67 in the xenograft tumor tissues. The groups were listed as in (A). C Schematic illustration of the regulatory mechanism of two ANLN splicing isoforms in the promotion of HNSCC progression.