Table 2.
Relative expression of VEGFa and VEGFb mRNAs in mouse macrophages.
| qPCR method | Time | mRNA | CT ± SD (n) | Fold (2−ΔΔCT)* |
|---|---|---|---|---|
| SYBR | 2 h | Rpl32 | 18.16 ± 0.71 (6) | |
| Vegfa | 30.75 ± 1.45 (7) | 1.00 | ||
| Vegfb | 25.43 ± 0.57 (6) | 32.72 | ||
| 8 h | Rpl32 | 19.12 ± 0.94 (5) | ||
| Vegfa | 33.92 ± 2.38 (7) | 1.00 | ||
| Vegfb | 28.45 ± 1.71 (4) | 36.30 | ||
| 24 h | Rpl32 | 17.82 ± 0.81 (5) | ||
| Vegfa | 32.07 ± 0.95 (5) | 1.00 | ||
| Vegfb | 26.07 ± 1.05 (5) | 52.34 | ||
| TaqMan | 0 h | Rpl32 | 18.19 ± 0.04 (4) | |
| Vegfa | 29.02 ± 0.34 (4) | 1.00 | ||
| Vegfb | 24.00 ± 0.17 (4) | 32.39 | ||
| 0.5 h | Rpl32 | 18.17 ± 0.10 (4) | ||
| Vegfa | 28.71 ± 0.44 (4) | 1.00 | ||
| Vegfb | 23.96 ± 0.15 (4) | 26.77 | ||
| 1 h | Rpl32 | 18.10 ± 0.03 (4) | ||
| Vegfa | 29.20 ± 0.10 (4) | 1.00 | ||
| Vegfb | 23.92 ± 0.30 (4) | 38.85 | ||
| 2 h | Rpl32 | 18.10 ± 0.06 (4) | ||
| Vegfa | 29.05 ± 0.22 (4) | 1.00 | ||
| Vegfb | 23.90 ± 0.07 (4) | 35.51 | ||
| 4 h | Rpl32 | 18.10 ± 0.10 (4) | ||
| Vegfa | 28.55 ± 0.39 (4) | 1.00 | ||
| Vegfb | 24.26 ± 0.14 (4) | 19.56 |
*Relative fold calculation for SYBR green qPCR: VEGFb/VEGFa mRNA levels = 2−ΔΔCT*68 bp (length of VEGFa amplicon)/83 bp (length of VEGFb amplicon).