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. 2021 Aug 3;11:15676. doi: 10.1038/s41598-021-95028-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-223 inhibits IL-18-induced NETs by suppressing calcium influx. (a) The levels of NETs in miR-223 over-expressing or miR-223 silencing cells after (a) IL-18 or (b) IL-1β treatment were detected and quantified. The calcium mobilization in miR-223 over-expressing cells after IL-18 treatment was detected by using Flou-4 staining and quantified with (c) confocal microscopy or (d) flow cytometry. All experiments were performed in triplicate, and data are presented as the mean ± SD. The scale bar in the IFA image represents 5 μm. **P < 0.01, ***P < 0.005.