Skip to main content
. 2021 Jul 21;12:723881. doi: 10.3389/fphar.2021.723881

FIGURE 2.

FIGURE 2

Changes in inflammatory indexes in vitro model of APAP induced by LPS/ATP combined with stimulation. (A,B) HMGB1 protein expression and quantitative analysis relative to GAPDH. (C,D) Immunofluorescence staining of HMGB1 and Caspase-1, the image is magnified 600×. (E) TUNEL staining, the image is magnified 400×. (F,G) Relative fluorescence quantitative analysis of C and D. # p < 0.05, ## p < 0.01, ### p < 0.001, significantly different from APAP group. *p < 0.05, **p < 0.01, ***p < 0.001, significantly different from the normal group. One-way ANOVA followed by Tukey’s test. The data are expressed as the mean ± SD of five independent assays with at least three replicates.