Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 21;12:723881. doi: 10.3389/fphar.2021.723881

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Liu, Jiang, Jin, Wu, Cui, Cui, Shang, Zhan, Lin, Jiao, Piao, Zhang, Sun, Nan and Lian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effects of neutrophils and NETs on liver injury induced by APAP. C57BL/6 mice were randomly divided into six groups: normal group, Ly6C/6G group, DNase I group, APAP group, APAP + Ly6C/6G group and APAP + DNase I group. Ly6C/6G (200 μg/mouse) was injected intraperitoneally 24 h before APAP (300 mg/kg) treatment. DNase I (10 mg/kg) was injected intraperitoneally 1 h after APAP treatment. (A) Morphology of mouse liver tissue samples. (B,C) HE and TUNEL staining. (D) Immunohistochemical staining of NE, MPO and HMGB1. (E) Liver weight index. (F,G) The level of ALT and AST in serum. (H) Immunofluorescence staining of frozen sections of NE and MPO. Green and red fluorescent label NE and MPO, orange yellow label NE and MPO dual expression. All images were magnified by 400×. Specific staining indicated by arrow. *p < 0.05, **p < 0.01, ***p < 0.001, significantly different from the normal group. One-way ANOVA followed by Tukey’s test. The data are expressed as the mean ± SD of five independent assays with at least three replicates.