Fig. 3.

Knockdown of circ_0030998 promoted the malignant behavior of lung cancer cells. (A) In accordance with the splice junction of circ_0030998, the sequences of circ_0030998 siRNAs were designed. (B) The silencing effect of circ_0030998 was also identified using qRT–PCR assay in A549 and H1299 cells after transfection with circ_0030998 siRNAs. The promoting effect of circ_0030998 knockdown by siRNAs on cell proliferation was identified through CCK‐8 (C), colony formation (D), and EdU staining assays (E) in A549 and H1299 cells. (F) Transwell assay was adopted to determine the changes in the abilities of cells to migrate and invade in si‐circ_0030998#1‐ or si‐circ_0030998#2‐transfected A549 and H1299 cells (scale bar, 200 μm). Data represent the mean ± SD from three independent experiments. Student’s t‐test with two biological dependent or independent replicates was used to determine statistical significance;**P < 0.01