Fig. 4.

hsa_circ_0030998 reduced Taxol resistance in Taxol‐resistant lung cancer cells. (A) qRT–PCR confirmed the overexpression of circ_0030998 in Taxol‐resistant A549 and H1299 cells. (B,C) CCK‐8 assay was applied to determine the influences of different concentrations of Taxol on the inhibition ratio of Taxol‐resistant A549 and H1299 cells after circ_0030998 overexpression. (D) Knockdown of circ_0030998 by siRNAs was identified through qRT–PCR assay in Taxol‐resistant A549 and H1299 cells. (E,F) After treatment with different concentrations of Taxol, the inhibition ratio of Taxol‐resistant A549 and H1299 cells was assessed by CCK‐8 assay after circ_0030998 knockdown. (G,H) The IC50 of Taxol was confirmed through CCK‐8 assay in Taxol‐resistant A549 and H1299 cells after circ_0030998 overexpression or knockdown. Data represent the mean ± SD from three independent experiments. Student’s t‐test with two biological dependent or independent replicates was used to determine statistical significance;*P < 0.05, ***P < 0.001