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. 2021 Jul 21;12:686502. doi: 10.3389/fphar.2021.686502

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Copyright © 2021 Zhao, Ran, Yang, Chen, Ji, Xu, Zhang, Sun, Liu, Zhou, Zhou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of bicyclol on autophagy. (A) P62 and LC3-Ⅱ protein levels in mice were detected by western blot. (B) Paraffin sections of liver tissue were stained with p62 (red) and counterstained with DAPI to visualize nuclei (blue) by immunofluorescence. Scale bar, 30 um. (C) Immunoblots for p62 and LC3-Ⅱ in AML12 cells. (D) Immunoblots for Beclin-1 in AML12 cells. *p < 0.05 vs. sham or Cr; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. BDL or TCA. Protein levels were normalized to levels of β-actin.