Fig. 4.

Combination of AZD5363 with doxorubicin regulates key pro‐apoptotic signaling pathways. (A) Cell lines were treated with either doxorubicin (3 μm), AZD5363 (20 μm), or a combination of both for 24 h. Western blot was performed using relevant antibodies, and β‐actin was used as a loading control. Results shown are representative of three independent experiments. (B) Immunofluorescence experiments were conducted in order to determine the effect of the previous treatments on p53 and FOXO1 subcellular localization. Scale bar = 45 μm. [Colour figure can be viewed at wileyonlinelibrary.com]