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Assessment of cytokine production in murine splenocytes stimulated with EG95 antigen in vitro. The splenocytes isolated from mice immunized with L. lactis strains or purified rEG95 protein were stimulated with 10 µg/ml of purified rEG95 or 5 mg/ml of ConA. Supernatants were collected after 72 h of stimulation. IFN-γ (A), IL-10 (B), and IL-4 (C) levels were measured by ELISA. Data are shown as mean ± SD of duplicate experiments (n = 5).
