Supplementary Fig. S1.

Identification of recombinant plasmid pNZ7021-eg95 and detection of the EG95 protein expression. (A) PCR amplification of eg95 fragment. Lane 1, PCR product from empty pNZ7021 vector (247 bp); lane 2, PCR product from recombinant pNZ7021-eg95 vector (1189 bp); lane 3, DNA ladder (100 bp). (B) Restriction digestion analysis of the pNZ7021-eg95 expression vector. Lane 1, DNA ladder (100 bp); lane 2, undigested pNZ7021-eg95 vector; lane 3, BglII digested pNZ7021-eg95 expression vector. (C) EG95 identity confirmation through Western blotting. Lane 1, protein MW marker; lane 2, purified rEG95-GST protein (43.5 kDa; positive control); lane3, recombinant L. lactis NZ9000 strain containing pNZ7021-eg95 vector (~33 kDa); lane 4, L. lactis NZ9000 strain containing empty pNZ7021 plasmid (negative control).