Skip to main content
. 2021 Jul 21;12:674016. doi: 10.3389/fimmu.2021.674016

Figure 5.

Figure 5

Chronic allo-antigenic stimulation promotes the generation of innate CD8 T-cell subsets and a senescent/inflammaging signature. (A) Frequencies of innate CD8 T-cell subpopulations are selectively increased in long-term kidney allograft recipients. Frequencies of CD8 T-cell subpopulations according to panKIR/NKG2A and/or Eomes expression among TCRαβ(+) CD8(+) live lymphocytes in healthy donors (HD, n=22) and long-term kidney allograft recipients (AlloTx, n=47). Representative plots are shown for one HD and one AlloTx recipient. (B–D) Senescent, EMRA and perforin(high) CD8 T-cell frequencies are increased in long-term kidney allograft recipients. Frequencies of senescent (CD27(-)CD28(-)) cells (HD n=15, AlloTx n=35) (A), EMRA (CD45RA(+) CCR7(-); HD n=13, AlloTx n=8) (B) and perforin(high)- expressing cells (HD n=22, AlloTx n=45) (C) in CD8 T-cell subpopulations. In the Conv E(-) cell pool from AlloTx patients, the increased frequency of EMRA cells ( Figures 5 and S4 ) could explain the relatively high frequency of senescent cells (19% vs 12% in healthy controls). Representative plots are shown for one HD and one AlloTx recipient in Figure S2 . Each dot represents one HD or AlloTx recipient. Two-tailed Mann-Whitney non-parametric test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. For detailed gating strategy, see Figure S1 .