Figure 2.

CECs in adult mice express Arg II and ROS and suppress IFN-γ production by T cell. (A) Representative flow cytometry plots, (B) cumulative data of percentages, and (C) absolute numbers of CD45+/CD45-CECs in the spleen of BALB/c male and female mice. (D) Representative flow cytometry plots, (E) cumulative data of percentages, and (F) absolute numbers of CD45+/CD45−CECs in the spleen of C57BL/6 male and female mice. (G) Cumulative data comparing percentages of CD45+CECs in spleens of BALB/c and C57BL/6 male and female mice. (H) Representative flow cytometry plot of PDL-1 and VISTA expression in CD45− and CD45+ CECs from a female BALB/c mouse. (I) Histogram plots, and (J) cumulative data of the mean fluorescence intensity (MFI) for ROS expression in CD45− and CD45+CECs in male and female BALB/c mice. (K) Cumulative data of MFI for ROS expression in CD45+ BALB/c male and female C57BL/6 mice. (L) Histogram plots, and (M) cumulative data of Arg II expression (MFI) in total CECs in male versus female mice compared to fetal liver CD71^low and CD71^high CECs. (N) Representative histogram plot of Arg I expression in splenic CD45+ and CD45−CECs of a female BALB/c mouse. (O) Representative flow plots, and (P) cumulative data of IFN-γ production by CD4+ T cells in the absence or presence of CECs at different ratios (CECs : CD4) after stimulation with anti-CD3/CD28 antibodies for 6 h. Unstimulated (Unstim), stimulated with anti-CD3/CD28 (stim). Each point represents data from an individual mouse, representative of at least three independent experiments. Bar, mean ± standard error.