Figure 3.

The single-copy orthologue of Deformed (Dfd) and Sex combs reduced (Scr) in P. opilio are necessary for the appendage identity of three body segments. (a) Wild-type HCR expression patterns of Dfd (green) and Scr (magenta) homologues in stage 11 and 15 embryos. (c) Wild-type (negative control) hatchling of P. opilio. (c) P. opilio hatchling from Po-Dfd RNAi treatment. (d) P. opilio hatchling from Po-Dfd + Scr RNAi treatment. (e–h) Appendage mounts of wild-type P. opilio hatchlings in lateral view. Insets: tarsal claws. (i–l) Appendage mounts of Po-Dfd RNAi hatchlings in lateral view. (m–p) Appendage mounts of Po-Dfd + Scr RNAi hatchlings in lateral view. (q) Schematic depiction of the mosaicism observed in hatchlings of Po-Dfd RNAi (Dfd KD) and Po-Dfd + Scr RNAi (Dfd + Scr KD) and distribution of phenotypic classes. Lighter colours indicate weaker penetrance. Only the two most frequent homeotic conditions are depicted (see electronic supplementary material, figure S5). Asterisk, reduced metatarsus; arrow, setal spurs; arrowhead, claw tooth; L1–L3: leg 1–3; tL1, fully transformed leg 1; ptL1–3: partially transformed legs 1–3; Mt, metatarsus; Pa, patella; Pp, pedipalp; Ta, tarsus; Ti; tibia. Scale bars: 100 µm. (Online version in colour.)