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The exhibition of embryos at the different stages using various techniques. Images of embryos at 18, 24, 42, and 66 h after insemination were presented. In the ICSI group and the conventional ICSI-AOA group, PN and cleavage cannot be observed in those oocytes, whereas, the oocytes treated by novel AOA can form pronuclei and develop to cleavage stage embryos normally. Scale bar = 50 μm.
