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. 2021 Jul 21;11:691762. doi: 10.3389/fonc.2021.691762

Figure 1.

Figure 1

HBO suppresses the hypoxia-induced Warburg effect in NSCLC cell lines via HIF-1α. (A) A549 and H1299 NSCLC cells were subjected to hypoxia (HypOx) with or without hyperbaric oxygen (HBO) and pcDNA.HIF1A transfection. (B) Glucose uptake in A549 and H1299 cells. (C) Intracellular pyruvate levels in A549 and H1299 cells. (D) Lactate secretion in A549 and H1299 cells. (E, F) Intracellular ATP levels in A549 and H1299 cells incubated (E) without or (F) with oligomycin A for 1 h. (G) Venn analysis identifies seven HIF-induced, upregulated glucose metabolism genes in hypoxic NSCLC cells. (H) qPCR analysis of expression of the seven glucose metabolism genes identified in (G) in A549 and H1299 cells. Peptidylprolyl isomerase A (PPIA) was used as the housekeeping gene. Data represented as means ± SDs. n = 3 biological replicates × two technical replicates. *P <0.05, **P <0.01 [one-way ANOVA].