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. 2021 Jul 30;16:5211–5232. doi: 10.2147/IJN.S319092

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© 2021 Gao et al.

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Characterization of the DMP-039/mRNA complex. (A) Size distribution of DMP-039 and mBim/DMP-039. (B) Zeta potential of DMP-039 and mBim/DMP-039. (C) Transmission electron microscopy (TEM) photomicrographs of DMP-039 stained by phosphotungstic acid solution (scale bar: 400 nm). (D) Cell viability assay of DMP-039 and PEI25K on 293T cells. (E) In vitro transcribed murine Bim mRNA (mBim) resolved by electrophoresis. (F) Gel retarding assay of the DMP-039/mRNA complex. (G) RNase protection assay of the mBim/DMP-039 complex.