FIGURE 1.

The oligodendrocyte lineage progression and expression of stage-specific markers. (A) Oligodendrocyte precursor cells (blue) differentiate into premyelinating oligodendrocytes (teal). A large majority of premyelinating oligodendrocytes are lost but some survive and integrate into neuronal circuits as new oligodendrocytes (pink). New oligodendrocytes then have the capacity to form myelin sheaths and slowly mature into myelinating oligodendrocytes (gray). (B) Oligodendrocyte precursor cells (blue) express Cspg4 and Pdgfra mRNA in high levels. Premyelinating oligodendrocytes (teal) have increased expression of Bmp4, Gpr17, Bcas1, and Enpp6. New oligodendrocytes (pink) express some of the same mRNAs as premyelinating oligodendrocytes, yet are distinct as they express Tmem2, Prom2, and Cx47 and have increased expression of myelin genes, such as Plp, Mobp, and Mag. Myelinating oligodendrocytes (gray) have high expression of myelin genes, such as Plp, Mobp, and Mag with little to no expression of Cspg4, Gpr17, and Enpp6 among others. Size of circles are relative to one another except for those labeled with “*” scale is given in black on the bottom row. The sizes of circles correlate with single cell RNA -sequencing data (Marques et al., 2016).