Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 21;12:707711. doi: 10.3389/fmicb.2021.707711

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Zhou, Huang, Liu, Xu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The histidine at the position of 191 in FleS is conserved with other histidine kinase and important in FleS signaling. (A) Sequence alignment of the HisKA domain of FleS with nine canonical HisKA domains from YycG (WP_023482385.1), PhoR (WP_003121316.1), FixL (WP_011119443.1), VgrS (WP_011038221.1), MtrB (WP_003917137.1), CzcS (WP_016253371.1), FimS (WP_003096404.1), PhoQ (WP_003082438.1), BqsS (WP_003090493.1). The arrow indicates the conserved histidine residue that is involved in phosphoryl group transfer. (B,C) The importance of the FleS H191 in regulating biofilm formation (B) and swimming motility (C) was examined by ectopic expression of FleS^H191A in the ΔfleS strain. ***P < 0.001, n.s., no significance (Student’s t-test). Representative images and the average diameter (D) of the swimming migration zone of each strain are shown. Each experiment was performed in triplicate.