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. 2021 Jul 21;12:707711. doi: 10.3389/fmicb.2021.707711

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Copyright © 2021 Zhou, Huang, Liu, Xu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FleR regulates flagellum biosynthesis independent of FleS. (A) Expression levels of flgB-E were examined in the PAO1 wild type (WT), the ΔfleS and ΔfleR mutants. N.D., not detected. (B) Observation of flagellum in the PAO1 wild type, the ΔfleS and ΔfleR mutants as well as their corresponding fleS and fleR complemented strains by TEM. A representative image of each strain is shown. (C) The average flagellum length measured from the stains in panel (B). Twenty cells were selected for the measurement from each strain.