FIGURE 2.

Kakonein inhibits NLRP3 inflammasome activation of cardiac vascular endothelium in hyperglycaemic mice. (A) Fluorescence indicating the effect of kakonein or metformin hydrochloride on NLRP3 (green) with caspase‐1 (red) co‐localization in cardiac endothelial cells. (B) Quantitative analysis of the co‐localization of NLRP3 with caspase‐1 (n = 8). (C) Fluorescence indicating the effect of kakonein or metformin hydrochloride on HMGB1 (green) with VWF (red) co‐localization. (D) Quantitative analysis of the co‐localization of HMGB1 with vWF (n = 8). (E) IL‐1β content in serum was detected by ELISA kit (n = 8). ^## P < .01 compared with the control. **P < .01 compared with hyperglycaemia