FIGURE 4.

Overexpressed miR‐515‐5p inhibited cell proliferation and promoted cell apoptosis. The HL60 and THP‐1 cells were transfected with miR‐515‐5p mimic or miR‐NC mimic. A, The cell viability of HL60 and THP‐1 cells was evaluated by CCK‐8 assay. B, The HL60 and THP‐1 cell proliferation was detected by Edu staining assay. C, Cell apoptosis in HL60 and THP‐1 cells was determined using flow cytometry assay. D, The Bax, cleaved caspase 3 and Bcl‐2 expression in HL60 and THP‐1 cells were detected by Western blot. E, The cell cycle distributions in HL60 and THP‐1 cells were determined using flow cytometry assay. **P < .01. Each experiment was repeated 3 times